Composite

Part:BBa_K1631001:Experience

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-12)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1631001

If you use this part, you can estimated the expression level of barnase and evaluate its toxic activity correspond to its expression level.

Usage and Biology

We succeeded to control growth rate of E coli.

We induced this parts and [Plac -rbs- GFP -d.term] with 100μM IPTG. [Plac -rbs- GFP -d.term] is negative control. We measured OD600 of 3 samples for each condition.

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We also evaluated the growth rate by slope of next graph.

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Plac GFP- 0.0213(/min)
Plac GFP+ 0.0205(/min)
Plac Barnase GFP- 0.0222(/min)
Plac Barnase GFP+ 0.0117(/min)


User Reviews

UNIQf18d91532ece22ee-partinfo-00000000-QINU UNIQf18d91532ece22ee-partinfo-00000001-QINU